spots
 
Ares lab Yeast Intron Database
Version 3.0
Created and maintained by Leslie Grate, Angela Telerski, Tyson Clark, Ross Centers and Manny Ares

Departments of Molecular, Cell & Developmental Biology and Computer Science in the Division of Natural Sciences and the Jack Baskin School of Engineering, University of California, Santa Cruz, CA  95064
Supported by the W. M. Keck Foundation, the Packard Foundation, and the National Institutes of Health.
Yeast Spliceosomal Introns

Intron Table

Extras

Links

Update History

Acknowledgments



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Santa Cruz

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New intron predictions that failed to splice


(tested using PCR primers specified in the Primers Table.)
ORF
NAME/FUNCTION
5' SPLICE SITE
BRANCH POINT
3' SPLICE SITE
BASE PAIRS
YBR057C
MUM2
GUAUGU
UAUUAAC
CAG
173
YDR318W
MCM21
GUAUGU
CACUAAC
AAG
111
YEL003W
GIM4
GUAUGU,GUAAGU,GUAUGC
UACUAAC
UAG
YHR039C
UNKNOWN
GUAUGU
CACUAAC
CAG
YHR124W
NDT80
GUAUGA
UACUAAC
AAG
<247
YKL133C
UNKNOWN
GUAUGU
CACUAAC
AAG
YML031W
NDC1
GUAUGC,GUAUGU
CACUAAC
CAG
340,263
YNL049C
UNKNOWN
GUAUGC
GGCUAAC
CAG,UAG
YOR180
ECI2
GUAUGA
GACUAAC
AAG
YOR195
SLK9
GUAUGA
UACUAAC
CAG
459
YPL208
unknown
GUACGU,GUACGU
UACUAAC
CAG

 

The above genes were identified by Carrie Davis as containing intron-like sequences.  Tests of intron splicing by RT-PCR were negative for these genes using the primer pairs indicated in the Primers Table.

















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